Global Advanced Research Journal of Microbiology (ISSN: 2315-5116) Vol. 4(11) pp. 124-129, December, 2015 Special Anniversary Review Issue

Copyright © 2015 Global Advanced Research Journals

Full Length Research Paper

Detection of Virulence Genes in Salmonella Isolated From Chicken and Chicken Waste Water

Nwiyi, Paul O¹, Soyoola Mobolalen² and Oguoma, Igwe O³

¹Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine Michael Okpara University of Agriculture, Umudike.

²Department of Veterinary Public Health and Preventive Medicine, Michael Okpara University of Agriulture Umudike.

³Department of Microbiology, College of Natural and Applied Science, Federal University of Science and Technology Owerri. Nigeria.

*Corresponding Author’s Email: afodiokechukwu@yahoo.com

Accepted 30 November, 2015

Abstract

The study was conducted in Aba, the commercial city of Abia State to determine the prevalence of Salmonella on chicken and chicken water waste using fecal samples from apparently healthy birds, clinically sick birds and swabs of waste water effluent from chicken farms. A total of 180 samples made of 60 samples each were collected from the three sample types. Biochemical and Serological analysis were conducted in veterinary laboratory of Michael Okpara University of Agriculture, while molecular studies were conducted at Lahor Medical Research and Diagnostic Center, Edo State Benin. The prevalence of Salmonella Spp. in apparently healthy chicken, clinically sick chicken and chicken water waste were 6.6% 11.6% and 20% respectively, while the mean prevalence rate was 12.7%. Polymerase chain reaction (PCR) was used for detection of virulence genes with known amplification conditions, number of cycles and concentration of reagents while amplicon were separated by electrophoresis in a 1.5% agarose gel. The detection of V_i and V₃ genes with bands at 100bp and 150bp suggest the presence of virulence genes of Salmonella Typhimurium, while the detection of inVA and sopE genes with bands at 260bp and 240bp indicates the presence of virulence genes for Salmonella Enteritidis.

Keywords: Salmonella, chicken samples, prevalence, virulence genes, amplification conditions.