Global Advanced Research Journal of Microbiology (Impact Factor (ISI) = 0.963)

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Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
June 2018 Vol. 7(4): pp. 064-072
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Paper

 

Isolation of Aerobic Bacteria from Wounds of Diabetic Patients Undergoing Hyperbaric Oxygen Therapy in Khartoum State

1*Ahmed Ibrahim Hashim, 1Amal Mubarak Hassan Mubarak, 1Nosiba Ahmed Hassan Hamed, and 2*Ahmed Bakheet Abd Alla

 

1*Department of Microbiology- College of Medical Laboratory Science, Sudan University of Science and Technology

2Department of Parasitology and Medical Entomology -College of Medical Laboratory Science, Sudan University of Science and Technology

Email: ahashima@gmail.com

 

Accepted 07 June, 2018

 

Abstract

Background: Diabetic wound infection is one of the complications of diabetes mellitus, hyperbaric Oxygen Therapy (HBOT) can be used as an adjacent treatment for diabetic wound infection, it involves inhalation of 100% oxygen under a pressure greater than 1 atmospheric absolute. This was a longitudinal case study, in which the wound specimens were collected from AL-Dirby Centre and further processing was done at the Microbiology Laboratory, Sudan University of Science and Technology (SUST), between January and July 2016. Objective: The aim of this study was to evaluate the effect of hyperbaric oxygen therapy (HBOT) on aerobic bacteria isolated from the wounds of diabetic patients attending Al-Dirby Center. Methodology: A total of 22 diabetic patients were involved in this study, their ages between 40 and 98 years. The majority of the patients were males 17 (77.3 %) and 5 (22.7%) were females, Insulin dependent diabetic patients were 15 (68.2%) and insulin independent diabetic patients were 7 (31.8%). A Questionnaire was used to collect data from the patients. The data was analyzed by using SPSS version (11.5). The results showed (78.1%) Gram-negative rods and (21.9%) Gram- positive cocci. The most frequent isolated bacteria were Proteus mirabilis 8(25%) followed by Pseudomonas aeruginosa 6(18.7%), Staphylococcus aureus and Escherichia coli 5 (15%), Proteus vulgaris 4(12.5%), and (Sterptococcus pyogenes, Serratia marcescens, Viridans streptococci, and Klebsiella pneumoniae) 1 (3.1%). Gram negative rods were more sensitive to Imipenem while Gram positive cocci were more sensitive to Gentamicin. Complete wound healing and bacterial eradication was observed in 17(77.3%) of the diabetic patients while incomplete wound healing and bacterial persistence was observed in only 5(22.7%). Conclusion: This study showed that HBOT could be a useful tool in woundstherapy.


Keywords:  Diabetic wound, hyperbaric Oxygen Therapy, AL-Dirby, Proteus mirabilis.

 

 

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Amal Mubarak Hassan Mubarak on Google Scholar Amal Mubarak Hassan Mubarak on Pubmed and Ahmed Bakheet Abd Alla on Google Scholar and Ahmed Bakheet Abd Alla on Pubmed Ahmed Ibrahim Hashim on Google Scholar Ahmed Ibrahim Hashim on Pubmed Nosiba Ahmed Hassan Hamed on Google Scholar Nosiba Ahmed Hassan Hamed on Pubmed