Global Advanced Research Journal of Educational Research and Reviews (GARJERR) ISSN: 2315-5132

November 2019, 8(1): pp. 001-005

Copyright © 2019 Global Advanced Research Journals

Full Length Research Paper

Additive Effect of Some Elements to vitamin B17 Administration in Immunosuppressed Mice Infected with Cryptosporidium

Soheir Mahmoud⁽¹⁾, Maisa Shalaby⁽¹⁾, Hoda Sabry⁽¹⁾, Neimat Amer⁽¹⁾,  Nevine Guirguis⁽¹⁾, ,Wafaa El Komy⁽¹⁾  Mennat  Al-Rahman ⁽¹⁾, and Mona Magdy⁽²⁾

Parasitology ⁽¹⁾ and Pathology ⁽²⁾ Departments, Theodor Bilharz Research Institute

Corresponding Author Email: nevinenessimtadros@gmail.com     

Accepted 19 October, 2019

Abstract

Cryptosporidiosis is known to trigger neoplastic gut changes in experimental animals. In this study, the efficacy of combined vitamin B17 (a newly introduced anticancer agent), and some elements such as vitamin B12, Zinc or Selenium, was tested in immunosuppressed Cryptosporidium infected mice. The aim of this work is to check whether the addition of either of these three elements solely to vitamin B17, could alleviate, or even hinder its deleterious effects in experimental models or not. A group of sixty (60) albino mice was used in the experiment. This group was further subdivided into ten subgroups. Subgroup1: immunocompetent infected mice. (100 Cryptosporidium oocysts four times per week for one month) Subgroup 2: immunocompetent infected mice treated with vitamin B17 (100 mg/kg b wt) 3weeks post infection, 3 times a week for 5 successive weeks. Subgroup 3: immunosuppressed infected mice. Subgroup 4: immunosuppressed infected mice treated with vitamin B17 (100 mg/kg) 2 weeks post infection, 3 times a week for 5 successive weeks. Subgroup 5: immunosuppressed infected mice treated with vitamin B17 (100 mg/kg) simultaneously with the infection 3 times a week for 5 successive weeks. Subgroup 6: immunosuppressed infected mice treated with vitamin B17 (100 mg/kg) 2 weeks prior to infection as a prophylactic dose, 3 times a week for 5 successive weeks. Subgroup 7: immunosuppressed infected mice treated with vitamin B17 (100 mg/kg) 3 weeks post infection, 3 times a week for 5 successive weeks. Subgroup 8: immunosuppressed infected mice treated with vitamin B17 (100 mg/kg) 3 weeks post infection, 3 times a week for 5 successive weeks, combined with vitamin B12 (0.57 mg/kg). Subgroup 9: immunosuppressed infected mice treated with vitamin B17 (100 mg/kg) 3 weeks post infection, 3 times a week for 5 successive weeks, combined with Zinc (25 mg/kg). Subgroup 10: immunosuppressed infected mice treated with vitamin B17 (100 mg/kg) 3 weeks post infection, 3 times a week for 5 successive weeks, combined with Selenium (50 mg/kg). Immune suppression was performed by using Dexamethasone orally in a dose of 0.25 mgm/kg/day for 4 successive days prior to inoculation with Cryptosporidium oocysts. Criteria of assessment included .parasitological oocysts count both in intestine and stools of infected animals. Histopathological studies included intestinal sections of all animal groups. It was found that co-administration of vitamin B17 with vitamin B12, Zinc or Selenium solely, revealed significant drop in the number of oocytes in immunosuppressed infected treated groups. As regards the histopathological findings, several degrees of inflammatory changes were seen in the infected untreated group such as villous atrophy, crypt elongation and mixed inflammatory cell infiltration in the laminapropria . These histopathological changes improved in the groups treated with B17 co- administered with vitamin B12, Zinc or Selenium solely. However, the improvement was more salient in the Zinc co-treated group.

Keywords: Vitamin B17, Cryptoporidiosis, Gut Cancer, Vitamin B12, Selenium and Zinc