Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094

January 2015 Vol. 4(1): pp. 026-032

Copyright © 2015 Global Advanced Research Journals

Full Length Research Paper

Potential Activity of Some Biofertilizer Agents on Antioxidant and Phytochemical Constituents of Faba bean Plant

Safinaz A. Farfour^1,4, Mahmoud A.Al-Saman^2,4Ragaa A. Hamouda^3,4

1Environmental Biotechnology Department,

2Industrial Biotechnology Department

3Microbial Biotechnology Department, 

4Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

*Corresponding Author’s Email: farfour.safinaz@yahoo.com  

Accepted 18 January, 2015 

 Abstract

Faba bean (Viciafabae L.,) is an annual legume crop which is consumed as plant foods for human and animal nutrition. So that, Rhizobium leguminosarum was used as nitrogen fixer with Aphanocapsaalpida and Laurenciaobtusa as biofertilizer agents to enrich faba bean plants with primary and secondary phytochemical content especially: nitrogen, protein, flavonoids, anteoxidants and polyphenolics contents. The results in this study showed that application of Aphanocapsaalbidacaused the highest increase of polyphenolic content in the root of faba bean plants followed by the treatment of Rhizobium leguminosarum var., fabae+ Laurenciaobtusa (10.78,7.97 and 7.54 mg Gallic acids /gm. dry wt. respectively), while the treatment of Laurenciaobtusa + Aphanocapsa albida gave the highest increase in polyphenolic content in the shoots of faba bean plants in compared with the other treatments and control (6.11 mg Gallic acids/gm. Dry wt.). All the treatments caused a significant increase in the shoots and roots antioxidants activity but Aphanocapsaalbida was the superior one. Rhizobium leguminosarum var.faba, caused the highest increase in  flavonoids contents in the shoots of faba bean plants followed by Rhizobium leguminosarum var. Faba + Laurenciaobtusawhereas the combination of different treatments caused the highest amounts of flavonoidsin the roots followed by the treatment of Rhizobium leguminosarum var.faba+Laurenciaobtusa. All the treatments caused increase in the both of shoots and roots protein content. The treatment Laurenciaobtusa + Aphanocapsaalbidacaused the best result which reached 12.5% and 11.87% in roots and shoots respectively followed by the treatment of Aphanocapsaalbida which caused 11.87% in the both of roots and shoots of faba bean plants.

Keywords: faba bean, phytochemicals content, antioxidant, polyphenole, Tannic acid, flavonoids, protein contents.