Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
May 2016 Vol. 5(6): pp. 057-067
Copyright © 2016 Global Advanced Research Journals

Full Length Research Paper

Distribution of free-living amoebae in springs in Morelos, Mexico.

Elizabeth Ramirez¹, Esperanza Robles¹, Maria E. Martinez¹, Maria G. Sainz¹, Blanca Martinez¹, Blanca I. Rivas¹ and Arturo Rocha²

¹Proyecto de Conservación y Mejoramiento del Ambiente. Facultad de Estudios  Superiores Iztacala. Universidad Nacional Autónoma de México. Avenida de los Barrios 1, Los Reyes Iztacala. Tlalnepantla 54090 Estado de México. México.

²Laboratorio de Ecología, Facultad de Estudios Superiores Iztacala. Universidad Nacional Autónoma de México. Avenida de los Barrios 1, Los Reyes Iztacala. Tlalnepantla 54090 Estado de México. México.

Email: erf@unam.mx

Accepted 16 August, 2016

Abstract

Free-living amoebae (FLA) can accomplish their life cycle as free-living organisms; therefore it is important to know more about the distribution of FLA in the environment. The aim of the study was to determine the presence and distribution of free-living amoebae in recreational and drinking water springs. Eight springs were selected and the samples were taken on a bimonthly basis for one year. Filtration of water samples was performed and culture was done on non-nutrient agar medium with Enterobacter aerogenes bacteria. Identification of FLA was carried out taking in account the morphological characteristics of the trophozoite and cyst. Forty one of 48 (85.4%) samples tested positive for the presence of free-living amoebae and isolated amoebae belonging to 15 genera. Genera were grouping into 4 groups in according to spatial-seasonal distribution, having Naegleria and Vermamoeba the highest frequency and distribution. The study contributed to knowledge of the diversity of free-living amoebae in springs and provided interesting information about the spatial and seasonal distribution FLA in these water bodies. 

Keywords: free-living amoebae, Naegleria, Vermamoeba, springs, recreational water.